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. 2015 Mar 2;14(3):206–215. doi: 10.1128/EC.00214-14

FIG 1.

FIG 1

α-KDE1 is essential in BF T. brucei. Effect of α-KDE1 RNAi knockdown on the growth and morphology of BF T. brucei. (A) Growth of α-KDE1 RNAi T. brucei cells in culture at 37°C in the presence (+) or absence (−) of doxycycline. (B) Northern blot analysis of the levels of α-KDE1 and β-tubulin mRNAs. Total cell RNA was isolated following induction with doxycycline, fractionated on agarose gels, and hybridized with specific radioactively labeled probes for α-KDE1 and tubulin. (C) DIC images taken from videos of α-KDE1 RNAi T. brucei cells following induction with doxycycline for 0, 6, 12, and 18 h. The position of the expanding posterior vacuole is indicated (arrow). (D) SEM of α-KDE1 RNAi T. brucei following treatment with doxycycline. The position of the flagellum (f) is indicated.