FIG 6.

Localization of α-KDE1 to the glycosome of BF T. brucei. α-KDE1 was tagged with a C-terminal HA epitope and used to prepare a constitutively expressing α-KDE1–HA cell line. (A) Total cell protein from wild-type (WT) and α-KDE1–HA (E1) cells was fractionated by SDS-PAGE and analyzed by Western blotting. On the left is the Coomassie blue-stained gel, and on the right is the blot following incubation with anti-HA antibody. The values to the left are molecular sizes in kilodaltons. (B, C) Localization of α-KDE1–HA by immunofluorescence microscopy relative to the mitochondrion stained with MitoTracker (B) and aldolase (C). The positions of the nucleus (N) and kinetoplast (K) are indicated.