Table 1.
Gene target | Primer name | Sequence | Annealing temperature | Amplicon size (bp) |
---|---|---|---|---|
TEM | TEM-F TEM-R |
5′-TCCGCTCATGAGACAATAACC-3′ 3′-ATAATACCGCACCACATAGCAG-5′ |
58 | 296 |
SHV | SHV-F SHV-R |
5′-TACCATGAGCGATAACAGCG-3′ 3′-GATTTGCTGATTTCGCTCGG-5′ |
60 | 450 |