Figure 7. Inheritance of cell cycle time during late divisions.

(a) The duration of mAG⁺, mKO2⁺, DN phases was measured from time-lapse imaging of each generation. Data are summarized from three independent experiments. The bar graphs represent means with s.d. Cells from the 8th generation were further grouped into ‘early divider’ (divided on the 1st day of imaging) and ‘later divider’ (divided on the 2nd day of imaging). Data were summarized from three independent experiments from Fig. 4. (b) Fractions of daughters grouped according to their cell cycle time (fast<600 min, 600 min<medium<800 min, slow>1,000 min and no division >30 h until the end of imaging) are shown. Each bar represents the cells from the sorted 1st, 3rd or 8th generation. Data were summarized from three independent experiments from Fig. 4. (c) Inheritance of cell cycle time from mothers to daughters. Fractions of daughters derived from the mothers grouped according to cell cycle time (fast<600 min, 600 min<medium<800 min, slow >1,000 min and no division >30 h until the end of imaging) are shown. Data are summarized from three independent experiments from Fig. 4. Significance value for the ‘slow’ and ‘no division’ fractions of each generation were calculated using the χ²-test (P<0.001).