Table 1.
Primary and Secondary Antibodies
| Primary antibody | Corresponding secondary antibody | ||||
|---|---|---|---|---|---|
| Antibody | Dilution | Company catalog No. | Antibody | Dilution | Company catalog No. |
| Mouse monoclonal | 1:200 | Millipore | Goat Cy3 | 1:500 | Jackson |
| anti-nestin | MAB353 | anti-mouse | 115-165-068 | ||
| Rabbit polyclonal | 1:100 | BTI | Goat dylight 488 | 1:500 | Jackson |
| anti-laminin | BT-594 | anti-rabbit | 111-485-144 | ||
| Mouse monoclonal | 1:50 | Abcam | Goat Cy3 | 1:200 | Jackson |
| anti-pan cadherin | Ab6528 | anti-mouse | 115-165-068 | ||
| Rabbit polyclonal | 1:200 | Dako | Goat Cy3 | 1:500 | Jackson |
| anti-S100 | Z0311 | anti-rabbit | 111-165-144 | ||
| Mouse monoclonal | 1:50 | Covance | Goat Cy3 | 1:50 | Jackson |
| anti-neuronal class III β-tubulin | MMS-435P | anti-mouse | 115-165-068 | ||