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. 2014 Dec 29;47(3):45–57. doi: 10.1152/physiolgenomics.00037.2014

Fig. 5.

Fig. 5.

qRT-PCR validation of miR-30b and miR-30c regulated targets in human skeletal muscle cells. A–H: negative control miRNA mimic (Scr, white bar), miR-30b (gray bar), or miR-30c (black bar) were overexpressed in skeletal muscle cells and expression of predicted targets were determined by qPCR. Expression of predicted target genes of cells transfected with miR30b or miR30c are expressed as fold change to Scr. Data are means ± SE (n = 3). *P < 0.05, **P < 0.01 by Student's paired t-test compared with Scr-transfected cells. I: overview of luciferase experiment setup with 3′-untranslated region (UTR) plasmids highlighting the putative binding sites of miR-30 isoforms. J: negative control miRNA mimic (Scr, white bar), miR-30b (gray bar), or miR-30c (black bar) were overexpressed in HEK293 cells together with indicated luciferase 3′-UTR plasmid. Luciferase activity was measured 24 h after transfection, and activity was expressed as fold change to Scr-transfected cells. Data are means ± SE (n = 3–6). *P < 0.001 by Student's t-test compared with Scr transfected cells.