Table 2.
Top five GO categories of biological process enriched by genes either up- or downregulated in human skeletal muscle cells after 4 days of differentiation
| GO Identity | GO Term | Genes, n | P Value | FDR |
|---|---|---|---|---|
| Upregulated genes | ||||
| GO:0006936 | muscle contraction | 29 | 1.4 × 10−18 | 2.4 × 10−15 |
| GO:0003012 | muscle system process | 29 | 1.8 × 10−17 | 1.6 × 10−14 |
| GO:0006941 | striated muscle contraction | 14 | 8.8 × 10−12 | 4.8 × 10−9 |
| GO:0007517 | muscle organ development | 24 | 1.4 × 10−10 | 6.2 × 10−8 |
| GO:0014706 | striated muscle tissue development | 15 | 2.4 × 10−7 | 8.5 × 10−5 |
| Downregulated genes | ||||
| GO:0007049 | cell cycle | 108 | 3.8 × 10−52 | 8.4 × 10−49 |
| GO:0000279 | M phase | 76 | 7.4 × 10−52 | 8.2 × 10−49 |
| GO:0022403 | cell cycle phase | 81 | 1.5 × 10−49 | 1.1 × 10−46 |
| GO:0000087 | M phase of mitotic cell cycle | 61 | 9.9 × 10−46 | 5.4 × 10−43 |
| GO:0000280 | nuclear division | 60 | 5.4 × 10−45 | 2.4 × 10−42 |
Enrichment analysis of differentiation-related genes annotated with GO terms demonstrates involvement of multiple molecular pathways and biological processes. Number of regulated genes (D4/D0) is reported. P values were derived using Fisher's exact test. FDR determined by Benjamini-Hochberg adjusted P value.