Table 3.
Number of miR-30 target sites in 3′-UTR of qPCR-validated miR-30 predicted genes downregulated during human skeletal muscle cell differentiation
| Conserved Sites |
Poorly Conserved Sites |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Target Gene | total | 8mer | 7mer-m8 | 7mer-1A | total | 8mer | 7mer-m8 | 7mer-1A | Total Context+ Score |
| ADAM19 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | −0.32 |
| ITGA5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | −0.14 |
| NAPG | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | −0.26 |
| PRDM1 | 3 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | −0.46 |
| RASAL2 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | −0.04 |
| SH2B3 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | −0.23 |
| SNAI1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | −0.25 |
| WNT5A* | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
Number of conserved and poorly conserved target sites and total context score of miR-30 in the 3′-untranslated region (UTR) of miR-30 predicted target genes. Information obtained from the TargetScan database.
*
WNT5A is a pSILAC validated target of miR-30 (TarBase database) and has no predicted targets using TargetScan, while microRNA.org indicates 2 potential binding sites of miR-30 isoforms in the WNT5A 3′-UTR. N/A, not available.