Fig. 9.

Loss of Met and the loss of both Met and EGFR have a more detrimental effect on CCl₄-induced liver necrosis and regeneration than the loss of EGFR alone. A: livers were removed from mice and weighed at various times after a CCl₄ ip injection, and liver weight was expressed as a function of body weight. Note that the greatest loss of weight occurred for the animals that lost hepatocellular expression of both EGFR and Met. Loss of EGFR alone had no effect on liver weight. B: we monitored the necrotic area of the liver. Note that the greatest necrosis occurred for the animals that lost expression of both EGFR and Met. Loss of EGFR alone had no effect on liver necrosis. C: we evaluated cell proliferation by monitoring nuclear expression of Ki67 by immunohistochemistry at 48 and 120 h after CCl₄ injection. Note that the greatest delay in Ki67 synthesis occurred for the animals that lost expression of both EGFR and Met. Loss of EGFR alone had no significant effect on cell proliferation as judged by this marker. *P ≤ 0.002; **P ≤ 0.001.