Fig. 4.

The MLCK activity of IKK2 is coprecipitated with NF-κB essential modulator (NEMO). A and B: HVSMCs were treated with TNF-α (10 ng//ml) for 5 min. IKK complex was immunoprecipitated with anti-NEMO and subjected to in vitro phosphorylation reaction. The products were then analyzed by Western blot. The representative result (A) and summary (B) of 3 independent experiments are presented. *P < 0.05 vs. control, one-way ANOVA. C and D: cell lysates were prepared from wild-type and IKK^−/− mouse aortic smooth muscle cells. IKK complex was immunoprecipitated with anti-NEMO and subjected to in vitro phosphorylation reaction. The products were then analyzed by Western blot. The representative result (C) and summary (D) of 3 independent experiments are presented. *P < 0.05 vs. control, one-way ANOVA.