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. 2014 Dec 24;308(5):L464–L478. doi: 10.1152/ajplung.00278.2014

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Fig. 3. — A–C: immunoblots showing decreased vascular endothelial (VE)-cadherin protein in lungs of Eln^+/− compared with Eln^+/+ pups (A), quantitative assessment of pulmonary arterioles (20–100 μm diameter) (B) and smaller microvessels (<20 μm diameter) (C), predominantly capillaries (<10 μm diameter), showing less capillary surface density/100 alveoli in lungs of Eln^+/− compared with Eln^+/+ pups. Summary data, means and SD; n = 6/group; *significant difference, P < 0.05. D: images of Eln^+/+ and Eln^+/− lung sections (×400; scale bars 20 μm) stained with CD-31 antibody (brown), denoting discernible pulmonary capillaries (arrows). E: Eln^+/+ and Eln^+/− lung sections stained with α-smooth muscle (SM) actin antibody showed less medial smooth muscle thickness, expressed as a percent of vessel diameter, in pulmonary arterioles (<40 and 40–80 μm diameter) of Eln^+/− compared with Eln^+/+ newborn mice. Summary data, means and SD; n = 135 for Eln^+/+ <40-μm arterioles, n = 17 for Eln^+/+ 40- to 80-μm arterioles, n = 136 for Eln^+/− <40-μm arterioles, n = 44 for Eln^+/− 40- to 80-μm arterioles; *significant difference, P < 0.05.