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. 2015 Mar 3;5:8716. doi: 10.1038/srep08716

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Doubly deacetylated chitosan tetramers (A₂D₂) and pentamers (A₃D₂) were produced by deacetylating biotechnologically produced chitin pentamer and tetramer with NodB and COD in a combined reaction overnight (a). The resulting doubly deacetylated chitosan oligomers A₃D₂ and A₂D₂ were purified and separated from each other using SEC (b). Fractions containing A₃D₂ (c) and A₂D₂ (d) were pooled separately and analysed using UHPLC-ELSD-ESI-MS.