. 2015 Mar 3;5:8716. doi: 10.1038/srep08716

Table 2. Analysis of double deacetylation of GlcNAc_2–6 by a combined action of NodB and COD, analysed using UHPLC-ELSD-ESI-MS. GlcNAc_2–6 (1 mM) was incubated with 2.5 μM of both NodB and COD in ammonium hydrogen carbonate buffer (pH 8) at 37°C overnight.

	expected products			determined products NodB + COD
Subst.	M_w A_2–6	M_w A_1–5D₁	M_w A_0–4D₂	m/z	M_w
A₂	424.17	382.16	340.15	A₀D₂ (341.16 [M + H]⁺)	340.15
A₃	627.25	585.24	543.23	A₁D₂ (544.27 [M + H]⁺)	543.27
A₄	830.33	788.32	746.31	A₂D₂ (747.35 [M + H]⁺)	746.35
A₅	1033.41	991.40	949.39	A₃D₂ (475.73 [M + H]²⁺)	949.46
A₆	1236.49	1194.48	1152.47	A₄D₂ (577.28 [M + H]²⁺)	1152.56

Table 2. Analysis of double deacetylation of GlcNAc2–6 by a combined action of NodB and COD, analysed using UHPLC-ELSD-ESI-MS. GlcNAc2–6 (1 mM) was incubated with 2.5 μM of both NodB and COD in ammonium hydrogen carbonate buffer (pH 8) at 37°C overnight.