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. 2015 Mar 3;5:8686. doi: 10.1038/srep08686

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NDRG1 expression in MCF7-DNA, MCF7-WISP1-1, and MCF7-WISP1-2 cells was determined by western blot (A) and RT-qPCR (B). NDRG1 expression of MCF-7 cells after treatment with recombinant human WISP1 protein as determined by western blot (top) and RT-qPCR (bottom) (C). (D) The NDRG1 reporter vector containing the human NDRG1 promoter/enhancer DNA fragment (−4714 to +46) was co-transfected with different concentrations of WISP-1 expression vector into MCDF-7 cells. The luciferase activity of the NDRG1 reporter in MCF-7 cells was presented as the mean percentage ± SE (n = 6) in relation to no WISP-1 expression vector transfection group. (E) Relative luciferase activity of reporter vectors containing different fragments from the NDRG1 promoter/enhancer as indicated. Data are presented as mean percentage ± SE (n = 6) of the luciferase activity in relation to mock-transfected cells (*P < 0.01).