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. 2015 Mar 3;5:8686. doi: 10.1038/srep08686

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(A) The expression of NDRG1 in NDRG1-knockdown MCF-7 (MCF7-NDRG1si) cells and mock-knockdown MCF-7 (MCF7-COLsi) cells were determined by immunoblotting (top) and RT-qPCR (bottom). Data are expressed as mean ± SE (n = 3) and the NDRG1 level of MCF-COLsi cells is set as 1(*P < 0.01). (B) Cell proliferation of MCF7-COLsi (•) and MCF7-NDRG1si (○) cells was determined by the³H-thymidine incorporation assay. Each point on the curve represents the mean percentage ± SE (n = 4) in relation to Day 1. (C) The cell invasion ability of MCF7-COLsi and MCF7-NDRG1si cells after 48 hours incubation was measured using the matrigel-invasion assay. Experiments were performed in triplicate and repeated at least three times. Data of quantitative analysis are expressed as average cell counts/9 fields ± SE of MCF7-COLsi and MCF7-NDRG1si cells. (*P < 0.01).