Table 1.
Primers used during QRT-PCR. NT, nucleotide.
| Transcript | Primer Pair (5' to 3') | Amplified NT Location (Amplicon Size) | Accession Number |
|---|---|---|---|
| GAPDH | F: GCACCACCAACTGCCTGGCACCCTTG | 425–634 (210) | ENSGALT00000023323 |
| R: GGATGACTTTCCCCACAGCCTTAGCAGC | |||
| COL2A1 | F: CATCCTCATCCAGGGATCCAAC | 3533–3733 (200) | AF452711 |
| R: ACTCCTGATCGGCTCCGCCAATGT | |||
| SOX9 | F: AAATGACAGAAGAACAGGACAAATG | 293–585 (293) | ENSGALT00000038513 |
| R: CTTCACGTGGGGTTTGTTCTTG | |||
| PTHLH | F: TCAGAGCACCAGCTACTGCATGAC | 136–286 (151) | NM_205338 |
| R: CCTAAGCCTGCTACCAACACAA | |||
| PTH1R | F: GCACTAGAAACTACATCCACATGC | 521–754 (234) | DQ914925.1 |
| R: AGTAGTAATTGGTTGCCAGGAAGT | |||
| PTH3R | F: ACCAGCTGCCTCCCAGAATGGGAT | 111–204 (94) | XM_425837 |
| R: TGAAGTCGTAGATGTAGTCAGGGC | |||
| OPG | F: AGACTGGAACAGCAACGACGAG | 272–470 (199) | ENSGALT00000030811 |
| R: GACAGACTGCTTTGGATGACGT | |||
| RANKL | F: AGGAGGTGAAGTTAATGCCAGAAT | 101–398 (298) | ENSGALT00000027412 |
| R: AGTTTCCCATCACTGAACGTCATA |