Table A1.

Mean expression among treatment and bone combinations (least squares mean ± SE). Negative least squares means arise when the measured expression is highly variable among replicates and includes missing values for some replicates (particularly for OPG and RANKL).

Treatment Bone	Control			Perch during Laying			Perch during Pullet			Perch during Pullet and Laying
	F	P	T	F	P	T	F	P	T	F	P	T
SOX9	0.45 ± 0.99	4.29 ± 3.13	0.90 ± 0.74	1.65 ± 0.99	5.96 ± 3.13	0.59 ± 0.74	0.77 ± 0.99	1.41 ± 3.13	1.68 ± 0.74	1.75 ± 0.99	0.58 ± 3.13	1.71 ± 0.74
PTHLH	0.75 ± 0.69	1.30 ± 0.58	0.38 ± 0.84	1.77 ± 0.69	0.46 ± 0.58	0.64 ± 0.84	0.25 ± 0.69	0.55 ± 0.58	0.53 ± 0.84	0.10 ± 0.69	0.25 ± 0.58	1.67 ± 0.84
PTH1R	0.43 ± 0.15	1.52 ± 0.64	0.30 ± 0.75	0.38 ± 0.15	0.80 ± 0.64	1.25 ± 0.75	0.18 ± 0.15	0.24 ± 0.64	1.89 ± 0.75	0.14 ± 0.15	0.16 ± 0.64	0.40 ± 0.75
PTH3R	1.76 ± 0.68	2.99 ± 1.20	0.91 ± 0.67	1.85 ± 0.68	2.23 ± 0.64	1.41 ± 0.67	0.68 ± 0.68	1.28 ± 1.20	1.41 ± 0.67	0.64 ± 0.68	0.56 ± 1.20	2.31 ± 0.67
OPG	0.76 ± 1.09	0.18 ± 0.33	0.39 ± 0.39	0.24 ± 1.16	0.70 ± 0.39	0.19 ± 0.41	1.82 ± 1.03	0.76 ± 0.33	0.94 ± 0.37	1.42 ± 1.03	0.08 ± 0.32	0.68 ± 0.36
RANKL	1.09 ± 3.85	0.07 ± 0.09	0.23 ± 0.24	7.75 ± 3.84	0.34 ± 0.11	0.26 ± 0.30	−0.66 ± 4.50	0.25 ± 0.09	0.51 ± 0.25	0.45 ± 3.60	0.30 ± 0.09	0.53 ± 0.24
RANKL/OPG RATIO	0.23 ± 4.24	−0.04 ± 0.42	0.02 ± 0.53	9.73 ± 4.15	−0.62 ± 0.61	0.05 ± 0.73	−2.67 ± 4.77	−0.62 ± 0.40	0.36 ± 0.57	−1.49 ± 3.79	−0.19 ± 0.41	0.41 ± 0.51
COL2A1	0.92 ± 0.19	0.59 ± 0.11	0.68 ± 0.13	1.02 ± 0.19	0.45 ± 0.11	0.69 ± 0.13	0.53 ± 0.19	0.39 ± 0.11	0.51 ± 0.13	0.68 ± 0.19	0.74 ± 0.11	0.84 ± 0.13