Alignment of amino acid sequences of C. semilaevis mature FSHβ (A), LHβ (B) and CGα (C) subunits with those of representative vertebrates. The cysteine residues are marked by gray shadow and numbered from the N terminus. The N-linked glycosylation sites are indicated by black shadow. Sole: Cynoglossus semilaevis, Senegalese: Solea senegalensis, Halibut: Hippoglossus hippoglossus, Flounder: Paralichthys olivaceus, Seabass: Dicentrarchus labrax, Grouper: Epinephelus coioides, Seabream: Pagrus major, Mtilapia: Oreochromis mossambicus, Killifish: Fundulus heteroclitus, Goldfish: Carassius auratus, Zebrafish: Danio rerio, Trout: Oncorhynchus mykiss, Eel: Anguilla anguilla, Sturgeon: Acipenser baerii, Catfish: Clarias gariepinus, Stickleback: Gasterosteus aculeatus, Ntilapia: Oreochromis niloticus, Human: Homo sapiens.