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. 2015 Jan 27;16(2):2767–2793. doi: 10.3390/ijms16022767

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Relative mRNA expression levels in maturing female C. semilaevis tissues. The abundance of FSHβ (A), LHβ (B) and CGα (C) transcripts, respectively, was determined by qRT-PCR and normalized to 18S (n = 4). Each bar represents the mean ± SE. Tissues marked by the different letters differing significantly from each other (p < 0.05). B: Brain, P: Pituitary, G: Gill, H: Heart, HK: Head kidney, K: Kidney, L: Liver, S: Spleen, St: Stomach, I: Intestine, O: Ovary, M: Muscle.

Relative mRNA expression levels in maturing female C. semilaevis tissues. The abundance of FSHβ (A), LHβ (B) and CGα (C) transcripts, respectively, was determined by qRT-PCR and normalized to 18S (n = 4). Each bar represents the mean ± SE. Tissues marked by the different letters differing significantly from each other (p < 0.05). B: Brain, P: Pituitary, G: Gill, H: Heart, HK: Head kidney, K: Kidney, L: Liver, S: Spleen, St: Stomach, I: Intestine, O: Ovary, M: Muscle.