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Effect of Tiron (TIR) and diphenylene iodonium (DPI) on the CHT-induced O2•− (A) and H2O2 (B) accumulations in the culture medium. At the indicated times aliquots of culture medium were collected and O2•− and H2O2 content was measured. Mean values ± SD (n ≥ 9) are presented. Different letters indicate significant differences among treatments at each experimental time (Tukey HSD test, p ≤ 0.05).
