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. 2015 Jan 30;16(2):3148–3162. doi: 10.3390/ijms16023148

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

MiR-21 was upregulated and miR34b/c was down-regulated in breast cancer cells, tissues, and serums. (A) Relative expression levels of miR-21, miR-34b and miR-34c in 15 paired of breast cancer tumors (BCT) and non-malignant adjacent tissues (NT) were determined by RT-qPCR assay, and normalized to the U6 levels; (B) Scatter plots of the Spearman correlation coefficient (rho) corresponding to an inverted monotonic trend between relative miR-21, and miR-34b, or miR-34c levels in breast cancer tumors; (C) Levels of miR-21, miR-34b and miR-34 in MCF-10A, T47D, MDA-MB-231, and MDA-MB-468 cells were determined by RT-qPCR assay, and normalized to the U6 levels; and (D) Serum levels of miR-21, miR-34b and miR-34 in breast cancer patients and healthy people were analyzed by Taqman RT-qPCR assay. All results represent the mean ± SD from three independent experiments. ** indicate significant difference at p < 0.01, respectively.

MiR-21 was upregulated and miR34b/c was down-regulated in breast cancer cells, tissues, and serums. (A) Relative expression levels of miR-21, miR-34b and miR-34c in 15 paired of breast cancer tumors (BCT) and non-malignant adjacent tissues (NT) were determined by RT-qPCR assay, and normalized to the U6 levels; (B) Scatter plots of the Spearman correlation coefficient (rho) corresponding to an inverted monotonic trend between relative miR-21, and miR-34b, or miR-34c levels in breast cancer tumors; (C) Levels of miR-21, miR-34b and miR-34 in MCF-10A, T47D, MDA-MB-231, and MDA-MB-468 cells were determined by RT-qPCR assay, and normalized to the U6 levels; and (D) Serum levels of miR-21, miR-34b and miR-34 in breast cancer patients and healthy people were analyzed by Taqman RT-qPCR assay. All results represent the mean ± SD from three independent experiments. ** indicate significant difference at p < 0.01, respectively.