Figure 3.

AKT inhibition induces EGFR upregulation in mRNA level in a Fork-head box O (FoxO)-dependent manner. (A) PANC-1 cells were transfected with AKT shRNA and treated with AZD8055 for indicated hours, then relative fold change of EGFR mRNA were analyzed by RT-PCR. GAPDH was used as a blank control. Error bars represent as mean ± SD.; (B) PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.