Table 5.
Adaptor and primer sequences used in AFLP analysis.
| Primer | Sequence |
|---|---|
| Adapters | |
| EcoRI-adapter | 5'-CTCGTAGACTGCGTACC-3' |
| 5'-AATTGGTACGCAGTCTAC-3' | |
| MseI-adapter | 5'-GACGTGAGTCCTGAG-3' |
| 5'-TACTCAGGACTCAT-3' | |
| Pre-Amplification Primer | |
| EcoRI | 5'-GACTGCGTACCAATTC-3' |
| MseI | 5'-GATGAGTCCTGAGTAA-3' |
| Selective Amplification Primer | |
| E-AGA/M-CAG | 5'-GACTGCGTACCAATTCAGA-3' |
| 5'-GATGAGTCCTGAGTAACAG-3' | |
| E-AGA/M-CTA | 5'-GACTGCGTACCAATTCAGA-3' |
| 5'-GATGAGTCCTGAGTAACTA-3' | |
| E-AGG/M-CAC | 5'-GACTGCGTACCAATTCAGG-3' |
| 5'-GATGAGTCCTGAGTAACAC-3' | |
| E-ACC/M-CAG | 5'-GACTGCGTACCAATTCACC-3' |
| 5'-GATGAGTCCTGAGTAACAG-3' | |
| E-AGT/M-CTT | 5'-GACTGCGTACCAATTCAGT-3' |
| 5'-GATGAGTCCTGAGTAACTT-3' | |