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. 2015 Feb 16;16(2):4209–4225. doi: 10.3390/ijms16024209

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Characteristics of the identified unique phosphopeptides in different HPLC fractions and IMAC steps. (A) Distribution of identified unique phosphopeptides in different HPLC fractions; (B) Distribution of identified unique phosphopeptides in different IMAC steps; (C) Distribution of phosphorylated peptides based on their phosphorylation sites (p-sites) in different IMAC steps; (D) The pI value distribution of the identified phosphopeptides in different IMAC steps; (E) The hydrophobicity distribution of the identified phosphopeptides in different IMAC steps.