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. 2012 Mar 25;7(9):652–658. doi: 10.3969/j.issn.1673-5374.2012.09.002

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Copyright: © Neural Regeneration Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Amyloid precursor protein (APP) and vacuolar protein sorting 35 (VPS35) mRNA expression in PC12 cells (reverse transcription-PCR).

(A) APP and VPS35 mRNA expression in PC12 cells. In each group, APP expression is higher than that of VPS35. Aβ: Amyloid β protein; M: Marker; I-V: control, Aβ_25-35, 5, 10, 25 μM Schisandrin B groups, respectively.

(B) Quantification of APP and VPS35 mRNA expression. The data are expressed as mean ± SD of band absorbance ratio of target gene to β-actin, and analyzed by one-way analysis of variance and least significant difference method.

The experiments were repeated at least three times (n = 3). ^aP < 0.05, vs. control group; ^bP < 0.05, vs. Aβ_25-35 group. Furthermore, there were significant differences (P < 0.05) between the three Schisandrin B groups with different concentrations.