Amyloid precursor protein (APP) and vacuolar protein sorting 35 (VPS35) expression in PC12 cells (western blot method). I–V: control, Aβ25-35, 5, 10, 25 μM Schisandrin B groups, respectively.
(A) Western blot detection results of APP and VPS35 protein expression in PC12 cells. In each group, APP expression is higher than that of VPS35.
(B) Quantification of APP and VPS35 protein expression. The data are expressed as mean ± SD of the band absorbance value ratio of target protein to GAPDH, and analyzed by one-way analysis of variance and least significant difference method. The experiments were repeated at least three times (n = 3). aP < 0.05, vs. control group; bP < 0.05, vs. Aβ25-35 group. Furthermore, there were significant differences (P < 0.05) between the three Schisandrin B groups with different concentrations. Aβ: Amyloid β protein.