[Table/Fig-2]:

Overview of different non-viral vector delivery methods

Delivery methods	Key Mechanism	Tissue on which it is effective	Advantage	Disadvantage
Naked plasma/Plasmid DNA[p DNA] –Direct delivery	Endocytosis	Muscle, skin, liver, cardiac muscle and solid tumour.	Safety. Simplicity.	Low transfection efficiency.
Gene Gun	High pressure helium stream	Ovarian cancer cell line[invitro and in vivo preclinical model]	Flexibility. Low cytotoxicity. Good efficiency.	Shallow penetration
Electroporation	Enhancement of cell membrane permeability	Skin, muscle.	Good efficiency. Repeatable.	Tissue damage. Accessibility of electrodes to internal organ are limited.
Ultrasound + micro bubble	Enhancement of cell membrane permeability	Brain, cornea, kidney, peritoneal cavity, muscle, heart, vascular cells.	Safety. Flexibility.	Low efficiency.
Magnetofection	Pinocytosis and endocytosis	primary cells and cells difficult to transfect by other methods[only invitro]	Flexibility. Low cytotoxicity.	Transient transfection.
Inorganic molecules	Endocytosis	Invitro	Easy Production Storage stability. Surface functionalization.	Low efficiency
Type of vector	Key mechanism	Target tissue	Advantage	Disadvantage
Lipoplexes[Lipid based]	Endocytosis, DNA condensation,	Airway epithelial cells, endothelial cells, hepatocytes, muscle cells.	Safety Low cytotoxicity	Low to medium efficiency Some results immunogenicity.
Polyplexes and Dendrimers	Endocytosis, DNA condensation, protein sponge effect	Lung, oral cavity.	Low immunogenicity Fair efficiency	Complement activation Low efficiency Cytotoxicity.