Figure 4. Effect of in vivo exposure to CPE on priming by DCs.

DCs were purified from the skin-draining lymph nodes of BALB/c mice epicutaneously exposed to PBS, OVA, or OVA and CPE and cocultured with DO11.10 CD4⁺ T cells for 3 days, followed by restimulation with CD3/CD28. Data are mean ± SEM of 7 independent experiments, with each experiment consisting of 3 pooled mice per group. *P < 0.05, **P < 0.01.