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. 2014 Oct 20;124(11):4941–4952. doi: 10.1172/JCI76864

Figure 3. Endometrial VEGF overexpression leads to stimulation of placental sFLT1 production.

Figure 3

(A–F) Vegf and sFlt1 mRNA expression by ISH (A–D) on GD18 (sections in C and D adjacent to sections in A and B) and by qPCR on GD12, GD15, and GD18 in deciduas and placentas (E and F). Increases in the Vegf signal (arrows) in deciduas (B and E) and in the sFlt1 signal (arrowheads) in placentas (D and F) were evident in Endo-VEGF animals. (G–K) Similarly, significant increases in decidual VEGF protein expression were observed by Western blot of Endo-VEGF tissues (G and H). Corresponding increases in sFLT1 protein expression were found in the placenta (by Western blot; G and I) and in serum (by ELISA; K) of the same animals. No significant changes in circulating VEGF levels (bound plus free) were found by ELISA (J). Results are mean ± SD. *P < 0.05 (n = 5). Scale bars: 200 μm (A–D).