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. 2014 Oct 8;124(11):4953–4964. doi: 10.1172/JCI75456

Figure 6. In situ maturation of engrafted HLCs.

Figure 6

(A and B) Coimmunostaining for hALB, hAAT, and hAFP on liver sections of engrafted mice 100 days pe, visualized by confocal microscopy. Asterisks indicate central veins. Original magnification, ×10; scale bar: 400 μm. Images at ×20 magnification are shown in Supplemental Figure 6. (C) IFA for hALB, hAFP, and hAAT 14 days pe. Scale bar: 200 μm. (D) ELISA for hAFP in sera of engrafted mice during the first 2 weeks pe (black lines) and control nonengrafted mice (red line). (E and F) Coimmunostaining for hALB and human and mouse CYP450 isoforms, confirming expression of mature hepatic markers in engrafted HLCs. Scale bars: 200 μm. Asterisks indicate central veins.