Figure 2. RBP-J imposes a requirement for ITAM-mediated costimulation in osteoclast differentiation.

BMMs derived from control, Dap12^–/–, Rbpj^ΔM/ΔMDap12^–/–, Dap12^–/–Fcrg^–/–, and TKO mice were stimulated with RANKL for 5 days. TRAP staining was performed (A) and the number of TRAP-positive MNCs (≥ 3 nuclei per cell) per well was counted (B). TRAP-positive cells appear red in the photographs. Scale bar: 100 μm. Data are representative of at least 20 independent experiments. **P < 0.01. (C) Quantitative real-time PCR analysis of mRNA expression of Acp5 (encoding TRAP), Ctsk (encoding cathepsin K), and Itgb3 (encoding β3) in BMMs from control, Dap12^–/–Fcrg^–/–, and TKO mice treated with RANKL for the indicated times. Data are representative of at least 3 independent experiments. **P < 0.01.