Figure 3. RBP-J deficiency compensates for the defect of NFATc1, BLIMP1, and c-FOS induction in Dap12^–/–Fcrg^–/– cells by RANKL.

(A) Immunoblot analysis of NFATc1, BLIMP1, and c-FOS expression in whole-cell lysates obtained from control, Rbpj^ΔM/ΔM, Dap12^–/–Fcrg^–/–, and TKO BMMs at the indicated time points after stimulation with RANKL. GAPDH or β-tubulin was measured as loading control. Isoform A of NFATc1 is the major isoform induced during osteoclastogenesis. Data are representative of at least 3 independent experiments. Lanes separated by a black line indicate that they were run on the same gel but were noncontiguous. (B) Quantitative real-time PCR analysis of mRNA expression of Nfatc1 (encoding NFATc1), Prdm1 (encoding BLIMP1), and Fos (encoding c-FOS) induced by RANKL for 24 hours in osteoclastogenic cell cultures from the indicated BMMs. Data are representative of at least 3 independent experiments. **P < 0.01.