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. 2014 Oct 8;124(11):5013–5026. doi: 10.1172/JCI76412

Figure 1. Oncogenic role of NCoA2 in PIN.

Figure 1

(A) Scheme of conditional overexpression of NCoA2 (ROSA26 Lox-STOP-Lox; Flag-NCoA2OE/+). (B) IF and Western blot analysis of NCoA2 overexpression in prostate epithelium from wild-type and NCoA2PCOE/+ mice at 2 months of age. (C) Top: Western blot analysis of NCoA2 expression in the prostates from wild-type and NCoA2PCOE/+ mice, immortalized prostate epithelial cells, RWPE1, and prostate cancer cells including LNCaP, LNCaP-Abl, DU145, PC3, and 22Rv1. Bottom: NCoA2 expression in NCoA2PCOE/+ and wild-type mice. (D) qRT-PCR analysis of Ncoa2 expression in the prostates from NCoA2PCOE/+ mice and the intact or castrated wild-type mice. (E) H&E-stained sections of representative anterior prostate (AP), dorsolateral prostate (DLP), and ventral prostate (VP) at 14 months of age in wild-type and NCoA2PCOE/+ mice. Arrows indicate PIN (bottom). Scale bars: 20 μm (B); 50 μm (E).