Figure 4. Collagen deposition and pulmonary function are improved in a murine model of bronchiolitis obliterans.
(A–C) PFTs were performed at day 60 after transplant on anesthetized animals. Animals (n = 4/group) were artificially ventilated and (A) resistance, (B) elastance, and (C) compliance were measured as parameters of distress in lung function in animals receiving 5 × 106 splenocytes (S) in addition to BM. Error bars indicate SEM. (D and E) Collagen deposition within pulmonary tissues was determined with a Masson trichrome staining kit; blue indicates collagen deposition. (D) Representative images of collagen deposition observed in each treatment cohort (n = 8). Blue staining represents Masson trichrome–stained collagen. Original magnification, ×200. (E) Quantification of collaged deposition (n = 8) as a ratio of blue area to total area of tissue was performed with the analysis tool in Photoshop CS3. Representative data from 3 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.