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. 2015 Jan 30;29(3):411–420. doi: 10.1210/me.2014-1275

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Figure 2. — Inflammatory factors down-regulate the expression of genes of BCAA metabolism. A, Expression of BCAA metabolism genes in 3T3-L1 adipocytes treated on day 8 after differentiation for 24 hours with 1 nM TNFα. B, Expression of BCAA metabolism genes in 3T3-L1 adipocytes treated on day 8 after differentiation for 24 hours with varying concentrations of TNFα. C, Expression of BCAA metabolism genes in 3T3-L1 adipocytes treated on day 8 after differentiation for 24 hours with 1 nM of the indicated cytokine. D, Expression of Bcat2, Sdha, Bckdha, Atp5a, and β-actin in 3T3-L1 adipocytes treated for 24 hours on day 8 after differentiation with 1 nM TNFα. E, Expression of BCAA metabolism genes in 3T3-L1s treated on day 8 after differentiation for 24 hours with 1 nM TNFα and 10 μM Bay11–7085. $, P < .05 compared with TNFα treatment. ND, not detectable. Error bars represent SEM. *, P < .05 compared with control (n = 6 per treatment group).