ER stress down-regulates BCAA metabolism genes. A, 3T3-L1 adipocytes were treated for 24 hours with 5 μM tunicamycin, 1 μM thapsigargin, or dimethylsulfoxide (DMSO), and the expression of BCAA/TCA genes were analyzed. B, Expression of spliced XBP1 in epididymal and subcutaneous fat of wild-type and FIXs mice. Expression of BCAA/TCA metabolism genes in wild-type and transgenic mice overexpressing spliced XBP1 maintained on a chow diet in the inguinal (C) or epididymal (D) depot are shown. Error bars represent SEM. *, P < .05 (n equals 4–8 per group).