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. 2015 Jan 16;29(3):396–410. doi: 10.1210/me.2014-1249

Figure 3.

Figure 3.

Knockdown of ME2 protein as judged from immunoblot analysis. There was 24 μg whole-cell protein per lane. A, Immunoblot of ME2 protein levels in control and single- and double-knockdown cell lines indicated. Immunoblotting did not detect ME2 protein in the Me2 double-knockdown cell lines Me2-2124(H)/Me2-2124(P) and Me2-2124(H)/Me2-654(P), and only a trace of ME2 was detected in the single-knockdown cell line Me2-2124(H). B, Immunoblot of ME2 protein in CHS and double-knockdown cell lines indicated. The level of ME2 protein detected was found to depend on the resident shRNA. The blots were stripped and reprobed for the mGPD protein as an internal control to show equal loading of protein across the lanes. The intensity of ME2 protein is shown as a percentage of the CHS and normalized to the intensity of mGPD relative to CHS control to correct for variation in protein in each lane.