Table 1.

Primers and probes for molecular detection of the T. pallidum subspecies and human DNA control

Organism/Control	Primer or probe	Sequence (5′ to 3′)	Final concentration (nmol/L)	Gene target	Product (bp)
TP-endemicum	FP*	TTATTAACCCCGCGTACACC	200	tp0620	173
	Probe	CalRed610†-ATGCCCATGTAGGGAACATCGGAG-BHQ2‡	200
	RP§	GCCAAAGTAACGCTCAGACC	200
TP-pallidum	FP	CACTCCTGTGGGGAGTAGGA	300	tp0620	136
	Probe	FAM†-CGATTACCTGCATCGGCAGGGTC-BHQ1	300
	RP	GAGCTCCCCGTTGCCA	300
TP-pertenue	FP	AGTCCTGCTGCAACGGTAGTAC	200	tp0858	93
	Probe	Quasar705†-TGCTGCACGAAGAAGTGCGAAGG-BHQ3	200
	RP	CGTACACCGAACCTTTGTCTT	200
Internal control	FP	CCA AGT GTG AGG GCT GAA AAG	80	Human RNase P	80
	Probe	Quasar670†- CC CCA GTC TCT GTC AGC ACT CCC TTC-BHQ3	80
	RP	TGT TGT GGC TGA TGA ACT ATA AAA GG	80

^*Forward primer.

^†Fluorescent dye.

^‡BHQ, black hole quencher.

^§Reverse primer.