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. 2015 Jan 7;92(1):45–49. doi: 10.4269/ajtmh.14-0488

Table 1.

Comparison of Cryptosporidium detection between microscopy and qPCR

DFA (+) DFA (−)
qPCR (+) 78 29
qPCR Ct (median) 28 (IQR = 26.1–30.7) 32 (IQR = 28.4–32.5)
qPCR parasite count (median) 2.4 × 106 (IQR = 6.9 × 105–1.1 × 107) 1.7 × 105 (IQR = 1.1 × 105–2.4 × 106)
DFA oocyst count (median) 3.7 × 105 (IQR = 9.3 × 104–8.4 × 105) n/a
qPCR (−) 2 15
qPCR Ct (median) n/a n/a
qPCR parasite count (median) n/a n/a
DFA oocyst count (range) 1 × 104 – 4 × 104 n/a

DFA = direct fluorescent antibody; qPCR = real-time polymerase chain reaction; IQR = interquartile range.