Table 1.
Comparison of Cryptosporidium detection between microscopy and qPCR
DFA (+) | DFA (−) | |
---|---|---|
qPCR (+) | 78 | 29 |
qPCR Ct (median) | 28 (IQR = 26.1–30.7) | 32 (IQR = 28.4–32.5) |
qPCR parasite count (median) | 2.4 × 106 (IQR = 6.9 × 105–1.1 × 107) | 1.7 × 105 (IQR = 1.1 × 105–2.4 × 106) |
DFA oocyst count (median) | 3.7 × 105 (IQR = 9.3 × 104–8.4 × 105) | n/a |
qPCR (−) | 2 | 15 |
qPCR Ct (median) | n/a | n/a |
qPCR parasite count (median) | n/a | n/a |
DFA oocyst count (range) | 1 × 104 – 4 × 104 | n/a |
DFA = direct fluorescent antibody; qPCR = real-time polymerase chain reaction; IQR = interquartile range.