FIGURE 5.

miR164 and miR159 detection in fruit juices of different plant species by dot blot hybridization. Five microliter of fruit juice were spotted on membranes and the miRNAs were detected by hybridization assays. (A) Expression of miR164 in lemon and mandarin juice, and in crude extract of green and ripe stages of prickly pear cactus and tomato fruits. (B) Expression of miR159 in different developmental stages of tomato crude extracts. The tomato fruit stages used for the crude extracts are shown below the membrane.