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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Mol Microbiol. 2015 Jan 16;95(4):723–737. doi: 10.1111/mmi.12902

Figure 3. LytS independent lrgAB expression during planktonic growth.

Figure 3

A. Cultures of KB5019 (wildtype), KB5020 (ΔlytSR), KB5024 (lytSTAA), KB5024 (pML11) (lytS complement) were grown in TSB to late exponential phase, harvested, and the β-galactosidase activity (Miller Units) generated by the chromosomal PlrgAB-lacZ was measured. The data presented are the average of two biological replicates (n=2) with standard error of the mean indicated. A two-tailed Student T-test assuming equal variances was used to determine statistical significance compared to wildtype. *p<0.05 B. Cultures of UAMS-1 (wildtype), KB5010 (ΔlytSR), KB5014 (lytSTAA), KB8000 (ΔackA), KB5050 (ΔackA-ΔlytSR), KB5051 (ΔackA-lytSTAA), KB8001 (Δpta), KB5046 (Δpta-ΔlytSR), and KB5047 (Δpta-lytSTAA) were grown in TSB to late exponential phase and harvested. RNA was isolated from the cells and qRT-PCR was utilized to quantify lrgA transcripts relative to the wild-type strains. The data presented are the average of two biological replicates (n=2) with standard error of the mean indicated. A two-tailed Student T-test assuming equal variances was used to determine statistical significance compared to wildtype. *p<0.05, **p<0.005