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. 2015 Feb 14;14:20. doi: 10.1186/s12934-015-0203-y

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© Yarimizu et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of repeat number of the single amino acid between K and E on secretory activities. a) The poly-I, F, and M sequence was changed to 10–17, 8–17, and 10–17, respectively and the activities were compared with WT signal sequence. Data were shown as in Figure 2. b) RT-PCR of the sequenced clones for WT (RAK6205), M¹⁶ (RAK8772), F¹³ (RAK9383) and L¹³ (RAK10336). Amplified cDNA bands of yGLuc and the control ACT1 at 30, 35, 40 cycles are shown. c) Western blotting analysis of the supernatants from WT (RAK6205) and M¹⁶ (RAK8772) using anti-GLuc antibody.