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. 2015 Mar 2;208(5):581–596. doi: 10.1083/jcb.201406040

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© 2015 Viero et al.

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Figure 8. — Frequency changes of the three cliques are reversible. (left) Cells were starved for 24 h, and then 10% FBS was added. After 12 h of serum addition, polysomes obtained from serum-starved cells and after FBS readdition were adsorbed on the mica and imaged by AFM. (The data shown are from a single representative experiment out of three repeats. For the experiment shown, n = 484 and 496, for starvation and FBS readdition, respectively.) Galleries of unclassified HMW polysomes are shown. Bars, 100 nm. (top middle) Comparison between the distribution of the number of ribosomes per polysome in the control (before serum withdrawal) and in starved cells. (bottom middle) Comparison between the distributions of the number of ribosomes per polysome obtained before and after FBS readdition to the starved cells. (right) RSC was run, and the frequency of each clique was calculated in the three conditions.