Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 2;208(5):533–544. doi: 10.1083/jcb.201407105

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Lee et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Ydj1 and Ssa2 affect transcript localization for cell polarity. (A) Cells grown for 12 or 24 h (30°C). Bars: (top) 10 µm; (bottom) 200 µm. (B) The 1-h lateral growth of the hyphal tip was measured with an FITC-ConA pulse. Error bars indicate SEM. *, P < 0.01. (C) BNI1 or SPA2 transcripts (yellow) are localized. Blue, DNA. Cells are outlined in gray. Bar, 5 µm. (D) BNI1 or SPA2 mRNAs were counted from each growing tip. Error bars indicate SEM. (D–F) n > 38 for each strain. (E) Overall mRNA density in mutants is compared with wild type. P > 0.24 for all strains. (F) Degree of clustering in mutants is compared with wild type. *, P < 0.01 by KS test. (G) Working model. Whi3 and Puf2 form complexes at new and preexisting sites of polarization (hyphal tips or nascent lateral branches) in the presence of polarity mRNAs.