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. 2015 Mar 2;208(5):613–627. doi: 10.1083/jcb.201408026

Figure 3.

Figure 3.

RSK regulates the interaction between K17 and hnRNP K. (A) Co-IP of K17 and hnRNP K. IP with anti-K17 antibody or PIS as a control was performed in A431 cells transiently transfected with RSK or NS siRNA. Immunoprecipitates (IP) or inputs were subjected to SDS-PAGE, and immunoblotting was performed with antibodies against the indicated proteins. (B) Co-IP of K17 and hnRNP K. A431 cells pretreated with 2.5 µM BI-D 1870, 1.44 µM SL0101, or DMSO as a control for 2 h were treated with 200 nM TPA or DMSO (vehicle control) for 15 min. IP with anti-K17 antibody or PIS as a control was performed. Immunoprecipitates (IP) or inputs were subjected to SDS-PAGE and immunoblotting was performed with antibodies against the indicated proteins. (C) Co-IP of K17 and hnRNP K. IP with anti-K17 antibody or PIS as a control was performed in A431 cells transiently transfected with WT or KI-RSK. Immunoprecipitates (IP) or inputs were subjected to SDS-PAGE and immunoblotting was performed with antibodies against the indicated proteins. (D) Co-IP of K17 and hnRNP K. A431 KRT17 shRNA cells were transfected with pEGFP-C3 vector, K17 WT, or S44A mutant. Cells were treated with 200 nM TPA or DMSO (vehicle control) for 15 min, and IP with GFP-Trap_A was performed. Immunoprecipitates (IP) or inputs were subjected to SDS-PAGE and immunoblotting was performed with antibodies against the indicated proteins.