Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 2;208(5):613–627. doi: 10.1083/jcb.201408026

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Chung et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — RSK regulates hnRNP K localization as well as the expression of CXCR3 ligands. (A) Immunostaining of hnRNP K (green) and K17 (red) in A431 cells transfected with RSK or NS siRNA. Nuclei are shown in blue. Bars, 10 µm. (B) A431 cells pretreated with 2.5 µM BI-D 1870 or DMSO as a control for 2 h were treated with 200 nM TPA or DMSO (vehicle control) for 1.5 h. mRNA levels of the indicated genes were measured using qRT-PCR. Data from three experimental repeats were normalized to DMSO control and are represented as mean ± SEM (error bars). *, P < 0.05. (C and D) A431 cells transiently transfected with RSK or NS siRNA were treated with 200 nM TPA or DMSO (vehicle control) for 1.5 h (C) or grown in normal growth condition (D). mRNA levels of the indicated genes were measured using qRT-PCR. Data from three experimental repeats were normalized to DMSO control and are represented as mean ± SEM. *, P < 0.05. (D) mRNA levels for the indicated genes from A431 cells transiently transfected with RSK or NS siRNA under basal condition were measured using qRT-PCR. Data from three experimental repeats (n = 6) were normalized to NS siRNA control and are represented as mean ± SEM. *, P < 0.05.