Table 1.
Gene | Direction | Oligonucleotide primer sequence |
shRNA-resistant K17a | Forward | 5′-GAGAATCTCAAGGAAGAACTAGCGTATCTGAAGAAGAACCACGAG-3′ |
Reverse | 5′-CTCGTGGTTCTTCTTCAGATACGCTAGTTCTTCCTTGAGATTCTC-3′ | |
GFP-K17b | Forward | 5′-GACTGGATCCAGCATGGTGAGCAAGGGCGAGG-3′ (BamHI restriction site underlined) |
Reverse | 5′-GCTGGGTCTAGATATCTCGAGTGTTAACGGGTGGTCTGGTGC-3′ (XbaI restriction site underlined) | |
GFPc | Reverse | 5′-GGTCTAGATTTACTTGTACAGCTCGTCCATGCCGAG-3′ (XbaI restriction site underlined) |
hnRNP Kd | Forward | 5′-CAGTCGACGATGGAAACTGAACAG-3′ (SalI restriction site underlined) |
Reverse | 5′-ATCCCGGGCTTAGAATCCTTCAAC-3′ (XmaI restriction site underlined) |
Used with a QuikChange II XL Site-Directed Mutagenesis kit.
Includes a BamHI or XbaI restriction site for ligation into pENTR1A plasmid (Invitrogen).
Used forward primer of GFP-K17b for PCR reaction.
Includes a SalI or XmaI restriction site for ligation into mCherry-C1 plasmid (Takara Bio Inc.).