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. 2015 Mar 2;208(5):613–627. doi: 10.1083/jcb.201408026

Table 1.

Oligonucleotide primers used in PCR reactions for cloning indicated genes into overexpression plasmids

Gene Direction Oligonucleotide primer sequence
shRNA-resistant K17a Forward 5′-GAGAATCTCAAGGAAGAACTAGCGTATCTGAAGAAGAACCACGAG-3′
Reverse 5′-CTCGTGGTTCTTCTTCAGATACGCTAGTTCTTCCTTGAGATTCTC-3′
GFP-K17b Forward 5′-GACTGGATCCAGCATGGTGAGCAAGGGCGAGG-3′ (BamHI restriction site underlined)
Reverse 5′-GCTGGGTCTAGATATCTCGAGTGTTAACGGGTGGTCTGGTGC-3′ (XbaI restriction site underlined)
GFPc Reverse 5′-GGTCTAGATTTACTTGTACAGCTCGTCCATGCCGAG-3′ (XbaI restriction site underlined)
hnRNP Kd Forward 5′-CAGTCGACGATGGAAACTGAACAG-3′ (SalI restriction site underlined)
Reverse 5′-ATCCCGGGCTTAGAATCCTTCAAC-3′ (XmaI restriction site underlined)
a

Used with a QuikChange II XL Site-Directed Mutagenesis kit.

b,c

Includes a BamHI or XbaI restriction site for ligation into pENTR1A plasmid (Invitrogen).

c

Used forward primer of GFP-K17b for PCR reaction.

d

Includes a SalI or XmaI restriction site for ligation into mCherry-C1 plasmid (Takara Bio Inc.).