Table 1.
Primers/probes and optimal concentrations.
| Serotype | Primer/probe sequences (5′–3′) | Position | Final conc. (μM/reaction) |
|---|---|---|---|
| DENV-1-F | ATCCATGCCCAYCACCAAT | 9865–9883 | 1 |
| DENV-1-R | TGTGGGTTTTGTCCTCCATC | 9945–9964 | 1 |
| DENV-1-Probe | FAM-TCAGTGTGGAATAGGGTTTGGATAGAGGAA-BHQ1 | 9907–9936 | 0.14 |
| DENV-2-F | TCCATACACGCCAAACATGAA | 9859–9879 | 1 |
| DENV-2-R | GGGATTTCCTCCCATGATTCC | 9963–9983 | 1 |
| DENV-2-Probe | FAM-AGGGTGTGGATTCGAGAAAACCCATGG-BHQ1 | 9916–9942 | 0.14 |
| DENV-3-F | TTTCTGCTCCCACCACTTTC | 9591–9610 | 1 |
| DENV-3-R | CCATCCYGCTCCTTGAGA | 9691–9708 | 1 |
| DENV-3-Probe | Cyan500-AAGAAAGTTGGTAGTTCCCTGCAGACCCCA-BHQ1 | 9633–9662 | 0.14 |
| DENV-4-F | GYGTGGTGAAGCCYCTRGAT | 9587–9607 | 1 |
| DENV-4-R | AGTGARCGGCCATCCTTCAT | 9744–9764 | 1 |
| DENV-4-Probe | Cyan500-ACTTCCCTCCTCTTYTTGAACGACATGGGA-BHQ1 | 9490–9519 | 0.14 |
| EAV-F | CATCTCTTGCTTTGCTCCTTAG | 1847–1868 | 0.2 |
| EAV-R | AGCCGCACCTTCACATTG | 1980–1997 | 0.2 |
| EAV-Probe | Cy5-CGCGCTCGCTGTCAGAACAACATTATTGCCCACAGCGCG-BHQ3 | 1926–1964 | 0.08 |