Figure 5.

Effects of cation chelators on seed mucilage morphology. Pontamine staining is shown following water or cation chelator treatment with gentle shaking of the wild type (WT) or various mutants as indicated. Whole-seed views (left column) and closeups of Pontamine-stained seeds (middle and right columns) are shown. Note the detachment of the outer cell wall remnants from the tips of the columella in cobl2 mutant seeds following treatment with the cation chelator EDTA. DIC, Differential interference contrast. Bars = 0.25 mm (left column) and 0.05 mm (middle and right columns).